Detection of nucleotide sequence changes by single-strand conformation polymorphism analysis.
نویسندگان
چکیده
منابع مشابه
Mutation Detection by Single Strand Conformation Polymorphism and Heteroduplex Analysis
Single strand conformation polymorphism (SSCP) and heteroduplex analysis (HDA) are two of the most popular electrophoresis-based mutation detection methods. Coupled to DNA amplification of the sequence to be analyzed, these techniques have become the methods of choice for a number of molecular diagnostic laboratories. This can be explained mainly by the numerous advantages, namely their technic...
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A new molecular biological approach for the identification of bacteria is described. This approach employs PCR of bacterial cell lysates with conserved primers located in the 16S rRNA sequence flanking a variable region, and analysis of the amplified product was based on the principle of single-strand conformation polymorphism (SSCP). The PCR product was denatured and separated on a nondenaturi...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 2001
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.45.205